SPARXS#

Papylio can be used for analyzing data from a Single-molecule Parallel Analysis for Rapid eXploration of Sequence space (SPARXS) experiment, as described in the research article Single-molecule structural and kinetic studies across sequence space and the protocol Single-molecule parallel analysis for rapid exploration of sequence space.

Obtaining the sequence characteristics from the data is divided into 6 step.

Single-molecule data analysis
Sequence data analysis
Aligning datasets
Coupling datasets
Single-molecule data analysis 2
Properties per sequence

When doing SPARXS for the first time or when using a new combination of microscope and sequencer, the global alignment will need to be determined using a separate experiment.

The documentation was made using the SPARXS example dataset which is freely available at Zenodo.